cd44-pe im7 antibody  (Santa Cruz Biotechnology)

Journal: Vaccines

Article Title: Type I Interferon Modulates the Function of Ly6C High-Expressing Naïve CD8 + T Cells to Promote an Antitumor Response

doi: 10.3390/vaccines13030246

Figure Lengend Snippet: The antibodies and reagents used in flow cytometry analysis. The antibodies used are shown, as well as their corresponding catalog number and company.

Article Snippet: CD44 Monoclonal Antibody (IM7), PE , 12-0441-82 , Thermo Fisher Scientific (San Diego, CA, USA).

Techniques: Flow Cytometry, Cell Isolation, Purification, Staining, Recombinant

Journal: Vaccines

Article Title: Type I Interferon Modulates the Function of Ly6C High-Expressing Naïve CD8 + T Cells to Promote an Antitumor Response

doi: 10.3390/vaccines13030246

Figure Lengend Snippet: Type I interferon preconditioning upregulates Ly6C expression on naïve CD8 T cells, enhancing effector functions. ( A ) Magnetic beads were used to isolate the naïve CD8 T cells from 6-week-old C57BL/6 (n = 3) or 6-week-old B6(Cg)-Ifnar1tm1.2Ees/J (n = 3) mice. Cells were incubated with 50 ng/mL of type I interferons IFNα or IFNβ1 for 24 h. The figure depicts the frequencies of Ly6C hi subsets in CD3 + CD8 + T cells for the indicated treatment groups. ( B – D ) To evaluate the effect of type I interferon on Ly6C expression in CD8 T cells, C57BL/6 mice (n = 5 per group) received a single dose of IFNβ (30 μg) or PBS control. PBMCs were collected on days 2, 7, 14, 21, and 28 and analyzed by flow cytometry. Representative gating of CD8 T cell subsets based on CD44 and Ly6C expression is shown for day 2 ( B ). The line graph illustrates the temporal dynamics of Ly6C⁺CD44⁻ ( C ) and Ly6C⁺CD44⁺ ( D ) CD8 T cell populations. ( E – G ) To evaluate the functional differences in Alb-IFNβ preconditioned CD8 T cells, naïve CD8+ T cells from 6-week-old C57BL/6 mice (n = 4) were isolated using magnetic beads and treated with 50 ng/mL Alb-IFNβ for 24 h, followed by stimulation with CD3 and CD28 antibodies for another 24 h. ( D – F ) The frequencies of CD69+ ( E ), IFNγ+ ( F ), and TNFα+ ( G ) in total CD8 T cells in C57BL/6 mice were examined across the specified treatment groups. In all circumstances, p -values ≤ 0.05 were considered significant (*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001).

Article Snippet: CD44 Monoclonal Antibody (IM7), PE , 12-0441-82 , Thermo Fisher Scientific (San Diego, CA, USA).

Techniques: Expressing, Magnetic Beads, Incubation, Control, Flow Cytometry, Functional Assay, Isolation

Journal: Frontiers in Immunology

Article Title: Anti-PD-L1 therapy altered inflammation but not survival in a lethal murine hepatitis virus-1 pneumonia model

doi: 10.3389/fimmu.2023.1308358

Figure Lengend Snippet: Flow cytometry antibody sources and concentrations.

Article Snippet: CD44 PE-Cyanine7 (clone IM7) , eBiosciences/Thermo Fisher , 25-0441-82 , 1.

Techniques: Flow Cytometry, Cytometry

Journal: eLife

Article Title: The lncRNA Malat1 inhibits miR-15/16 to enhance cytotoxic T cell activation and memory cell formation

doi: 10.7554/eLife.87900

Figure Lengend Snippet:

Article Snippet: Antibody , anti-CD44 PE-Cy7 (Clone-IM7, rat monoclonal) , eBiosciences , 25-0441-82 , (1:200).

Techniques: Enzyme-linked Immunosorbent Assay, Sequencing, CRISPR